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Issue:2 Citation: Snehal Funde et al. Ijppr

Snehal Funde, Swati Patil, Prin Kundnani
2017 unpublished

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Objective: To develop a sensitive, reliable, accurate and simplified quantitative high-performance thin layer chromatography (HPTLC) method and to determine the quantity of thymoquinone (TQ) in Nigella sativa seed extract (NSE). Methods: TQ (TQ) was separated on aluminum-backed silica gel 60 F254 plates (Merck Germany) with Toluene: Petroleum ether 8:2 (%, v/v) as mobile phase. Results: At R f value of 0.71±0.04 a compact band was observed. The linearity was in range of 10 to 40µg/spot of TQ
more » ... the correlation coefficient of 0.9987 indicated good linear dependence of peak area on concentration. Limit of detection (0.2 µg/spot), limit of quantification (0.4µg/spot) accuracy (less than 2) were found satisfactory. Conclusions: The developed HPTLC densitometric method was found to be cheap, selective, precise and accurate and can be used for routine analysis of NSE.
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Citation

Snehal Funde, Swati Patil, Prin Kundnani. "Issue:2 Citation: Snehal Funde et al. Ijppr." (2017)