Department of Chemical Engineering, Biochemical Engineering Division, Biotechnology Laboratory, University of Calcutta, 92, Acharya Prafulla Chandra Road, Kolkata-700 009, India E-mail : subhadeepgangulyphysiol@rediffmail.com Manuscript received 12 October 2011, revised 09 February 2012, accepted 22 February 2012 The production of L-glutamic acid with free cells of a mutant Micrococcus glutamicus AB100 was carried out using shake flask method through selection of suitable carbon and nitrogen

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... rces. Among 12 different carbon sources and 10 nitrogen sources tested, 9.0% glucose and 1.4% diammonium hydrogen phosphate were selected as most suitable carbon and nitrogen sources respectively for the maximum growth and L-glutamic acid production by this mutant. Production increased significantly (p < 0.01) from 10.4 to 14.8 mg/ml. Dry cell weight also increased significantly (p < 0.01) from 4.6 to 7.3 mg/ml.