Transplantation of metanephroi across the major histocompatibility complex in rats

Sharon A. Rogers, Helen Liapis, Marc R. Hammerman
2001 American Journal of Physiology. Regulatory Integrative and Comparative Physiology  
merman. Transplantation of metanephroi across the major histocompatibility complex in rats. Am J Physiol Regulatory Integrative Comp Physiol 280: R132-R136, 2001.-To determine whether transplanted metanephroi grow, differentiate, and function in hosts that differ in major histocompatibility complex loci (RT1 loci in rats) from donors in a defined way, we implanted metanephroi from embryonic day (E) 15 PVG (RT1 c ) rat embryos into the omentum of nonimmunosupressed uninephrectomized PVG-RT1 avl
more » ... omized PVG-RT1 avl (host) rats. By 4 wk posttransplantation, metanephroi had grown and differentiated such that glomeruli, proximal and distal tubules, and collecting ducts had normal structure and ultrastructure. At 12 wk posttransplantation, weights of metanephroi were 54 Ϯ 8 mg. Inulin clearances were 0.9 Ϯ 0.3 l ⅐ min Ϫ1 ⅐ 100 g rat wt Ϫ1 . In vitro, splenocytes from PVG rats stimulated the proliferation of cells originating from both PVG-RT1 avl rats in which a transplant had been performed and PVG-RT1 avl rats with no transplant. Full-thickness PVG-RT1 avl skin engrafted normally on PVG-RT1 avl rats in which PVG metanephroi had been previously implanted and metanephroi retained a normal appearance. In contrast, skin from PVG rats sloughed, and the tubular architecture of metanephroi was obliterated by a mononuclear cell infiltrate consistent with acute rejection. Here we show for the first time that functional chimeric kidneys develop from metanephroi transplanted across the MHC into nonimmunosupressed hosts and provide evidence that a state of peripheral immune tolerance secondary to T cell "ignorance" permits their survival.
doi:10.1152/ajpregu.2001.280.1.r132 pmid:11124143 fatcat:x2mub64ghvbnrepr2ntqm4anfy