This study evaluated the physicochemical and microbial quality of water samples from Epie creek, Niger Delta. Water samples were collected from five different locations (Akenfa, Agudama-Epie, Tombia, Opolo and Biogbolo) in two seasons viz: dry i.e. January and February and wet season i.e. May and June, 2016). The samples were analyzed following standard procedure. Results from the water quality ranged from 5.80-7.01 (pH), 0.12 mg/l-2.58 mg/l (salinity), 265.00 -1096.75 µS/cm (conductivity),

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... 9-105.04NTU (Turbidity), 133.00-548.50 mg/l (total dissolved solid), 3.74-10.562 mg/l(total suspended solid), 6.23-7.17 mg/l (dissolved oxygen), 65.17-94.95 mg/l (biological oxygen demand), 121.72-252.80 mg/l, (chemical oxygen demand), 6.504-14.30 mg/l (sulphate), 0.63-1.69 mg/l (nitrate), 2.18-4.28 mg/l (Bicarbonate), 15.25-61.75 mg/l (chloride), 19.20-62.00 mg/l (Alkalinity), 52.86-280.00 mg/l (Total hardness), 16.53-43.09 mg/l (calcium), 3.07-10.46 mgl (magnesium), 3.34-16.02 mg/l (sodium), 2.06-5.26 mg/l (potassium), 0.32-2.52 mg/l Original Research Article (iron), 0.016-0.188 mg/l (manganese), Analysis of variance showed that there was significance difference (P<0.05) in most of the location, months and interaction. The values showed that anthropogenic activities (mostly from marketing wastes in the creek is having an impact on the water quality).