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DNA Fiber Assay for the Analysis of DNA Replication Progression in Human Pluripotent Stem Cells
2020
Current protocols in stem cell biology
Human pluripotent stem cells (PSC) acquire recurrent chromosomal instabilities during prolonged in vitro culture that threaten to preclude their use in cell-based regenerative medicine. The rapid proliferation of pluripotent cells leads to constitutive replication stress, hindering the progression of DNA replication forks and in some cases leading to replication-fork collapse. Failure to overcome replication stress can result in incomplete genome duplication, which, if left to persist into the
doi:10.1002/cpsc.115
pmid:32584505
fatcat:546rk27x6zh7bk2srhmgcwnqie