Yeast TAF(II)90 is required for cell-cycle progression through G2/M but not for general transcription activation

L M Apone, C M Virbasius, J C Reese, M R Green
1996 Genes & Development  
The RNA polymerase II general transcription factor TFIID is a multisubunit complex comprising TATA-box binding protein and associated factors (TAFIIS). In vitro experiments have suggested that TAFIIS are essential coactivators required for RNA polymerase II-directed transcription activation. Here, for the first time, we analyze systematically the in vivo function of a specific TAFt, yeast TAFn90 (yTAFu90). We show that functional inactivation of yTAFH90 by temperature-sensitive mutations or
more » ... ve mutations or depletion leads to arrest at the G2/M phase of the cell cycle. Unexpectedly, in the absence of functional yTAF.90, a variety of endogenous yeast genes were all transcribed normally, including those driven by well-characterized activators. Taken together, our results indicate that yTAF.90 is not required for transcription activation in general, and reveal linkages between TAF n function and cell-cycle progression. [Downloaded from Access the most recent version at doi: 10: 1996, Genes Dev. L M Apone, C M Virbasius, J C Reese, et al. but not for general transcription activation. Yeast TAF(II)90 is required for cell-cycle progression through G2/M References http://genesdev.cshlp.org/content/10/18/2368.full.html#ref-list-1
doi:10.1101/gad.10.18.2368 pmid:8824595 fatcat:nxhe46qgbvckvdke46gup4mjm4