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DNA duplex and dumbbells containing chemically active acylphosphate internucleotide groups were synthesized. To obtain these compounds the chemical ligation method was used. The acylphosphate group was inserted into a DNA duplex and dumbbells as a result of template-directed condensation of 5Pphosphate and especially introduced 3P-carboxy groups of oligonucleotides. 1-Ethyl-3-(3P-dimethylaminopropyl)carbodiimide (EDC) was used as a condensing agent. Oligonucleotides containing a carboxy groupdoi:10.1016/s0014-5793(98)00812-6 pmid:9714562 fatcat:xwrltcowdbgnzpdvvmt6ddogmy