Mass spectrometric analysis of active ingredients in fertilized egg for lipid metabolism [post]

2020 unpublished
Adipose tissue is one of the important components of the animal body, and its development is restricted by various mechanisms. Yolk protein has an important effect on the development of chicken embryo skeletal muscle. The protein needed for chicken embryo development mainly comes from egg yolk. The protein metabolism in yolk is particularly strong after fertilization, and the nutrition level is much higher than that of ordinary eggs. In order to study which components in fertilized eggs play a
more » ... ilized eggs play a role in fat metabolism, Nano-HPLC-MS/MS protein detection was performed on fertilized egg extracts. A total of 295 proteins were identified and analyzed by Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and eukaryotic cluster (KOG)/homologous cluster (COG) analysis. The results indicate that metabolic pathways and PPAR signaling pathways are the main biological processes affecting lipid metabolism. Western Blotting (WB) was used to verify the possible components of fat metabolismrelated genes apolipo A-I (APOA1) and bile salt-activated lipase (BSAL, CEL). The results showed that APOA1 was highly expressed in fertilized eggs compared to unfertilized eggs. The expression of CEL was not significantly different between fertilized and unfertilized eggs. This study provides a theoretical basis for further studying the effects of fertilized eggs on biological metabolism.
doi:10.21203/rs.2.22228/v1 fatcat:nclkoknnmzdxjdcdkuoei3fc7m