Table S2: Case VS control 2 databases [component]

Background. Anorectal malformations (ARMs) are the most common congenital malformation of the gut, and regulated by multiple signal transduction pathways. The microRNA (miRNA) expression profiles and their biologial functions in anorectal malformations (ARMs) remain unclear. The aim of our study was to evaluate miRNA and mRNA expression profiles in the ARM rats. Methods and Materials. ARM was induced with ethylenethiourea (ETU) on gestational day 10. Cesarean deliveries were performed to
more » ... performed to harvest the embryos on gestional day 20. For the extraction of total RNA, 1 cm terminal hindgut samples were obtained from three fetal rats that had similar weights. The microarrays and quantitative RT-PCR analysis were conducted to evaluate the miRNA and mRNA expression profiles in normal fetal rats (n = 3) and ARM fetal rats (n = 3). Results. In total, 33 miRNAs and 772 mRNAs were significantly and differentially expressed in terminal hindgut tissues of ARM group versus control group. Functional annotation was performed to understand the functions and pathways of differentially expressed mRNAs. Also, we constructed a miRNA-target gene regulatory network including 25 differentially expressed miRNAs and 76 mRNAs. Furthermore, the credibility of the microarray-based results were validated by using qRT-PCR. Conclusion. The miRNA and mRNA expression in terminal hindgut tissue of ARM fetal rats might provide a basis for further research on the pathogenesis of ARMs. How to cite this article Xiao et al. (2018), Integrative analysis of microRNA and mRNA expression profiles in fetal rat model with anorectal malformation. PeerJ 6:e5774; Lewis BP, Shih IH, Jones-Rhoades MW, Bartel DP, Burge CB. 2013. Prediction of mammalian microRNA targets. Cell 115(7):787-798. Mattick JS, Makunin IV. 2006. Non-coding RNA. Human Molecular Genetics 1:R17-R29. Pichler M, Ress AL, Winter E, Stiegelbauer V, Karbiener M, Schwarzenbacher D, Scheideler M, Ivan C, Jahn SW, Kiesslich T, Gerger A, Bauernhofer T, Calin GA, Hoefler G. 2014. miR-200a regulates epithelial to mesenchymal transitionrelated gene expression and determines prognosis in colorectal cancer patients. British Journal of Cancer 110(6):1614-1621 DOI 10.1038/bjc.2014.51. Qin J, Luo M. 2014. MicroRNA-221 promotes colorectal cancer cell invasion and metastasis by targeting RECK. FEBS Letters 588(1):99-104
doi:10.7717/peerj.5774/supp-2 fatcat:azpig22ffzc5tjozipigxlnng4