High-resolution x-ray imaging for microbiology at the Advanced Photon Source

B. Lai
2000 AIP Conference Proceedings  
Exciting new applications of high-resolution x-ray imaging have emerged recently due to major advances in high-brilliance synchrotrons sources and high-performance zone plate optics. Imaging with subrnicron resolution is now routine with hard x-rays: we have demonstrated 150 run in the 6-10 keV range with x-ray microscopes at the Advanced Photon Source (APS), a third-generation synchrotrons radiation facility. This has fueled interest in using x-ray imaging in applications ranging fi-om the
more » ... nging fi-om the biomedical, environmental, and materials science fields to the microelectronics industry. One important application we have pursued at the APS is a study of the microbiology of bacteria and their associated extracellular material (biofilms) using fluorescence microanalysis. No microscopy techniques were previously available with sufficient resolution to study live bacteria (= 1~m x 4 pm in size) and biofilms in their natural hydrated state with better than part-per-million elemental sensitivity and the capability of determining g chemical speciation. In vivo x-ray imaging minimizes artifacts due to sample fixation, drying, and staining. This provides key insights into the transport of metal contaminants by bacteria in the " environment and potential new designs for remediation strategies. and sequeskation
doi:10.1063/1.1302783 fatcat:v2pxwmtvsffjng4dhxctggilpu