A novel bioassay for evaluating the efficacy of biocides to inhibit settling and early establishment of marinebiofilms [post]

Åsa Arrhenius, Thomas Backhaus, Annelie Hilvarsson, Ida Wendt, Aleksandra Zgrundo, Hans Blanck
2017 unpublished
This paper presents a novel assay that allows a quick and robust assessment of the effects of biocides on the initial settling and establishment of marine photoautotrophic biofilms. The assay integrates the response of the multitude of indigenous fouling organisms, which overcomes a major limitation of existing assays which are largely limited to testing only lab-cultivatable species. The assay was evaluated using eight antifouling biocides, for which full concentration-response curves are
more » ... nted. The efficacy ranking, based on EC 98 values from most to least efficacious compound is: copper pyrithione >TPBP >DCOIT >tolylfluanid>zinc pyrithione >medetomidine >copper (Cu 2+ ), while the ecotoxicological ranking (based on E C10 values) is irgarol, copper pyrithione >zinc pyrithione >TPBP >tolylfluanid >DCOIT >copper (Cu 2+ ) > medetomidine. The algaecide irgarol did not cause full inhibition. Instead the inhibition leveled out at 95% effect at 30 nmol l -1 , a concentration that was clearly lower than for any other of the tested biocides.
doi:10.7287/peerj.preprints.240v3 fatcat:l73qxf2hxjdwbna2doe7rhbfhm