Chitinilyticum litopenaei sp. nov., isolated from a freshwater shrimp pond, and emended description of the genus Chitinilyticum

S.-C. Chang, M.-C. Wu, W.-M. Chen, Y.-H. Tsai, T.-M. Lee
2009 International Journal of Systematic and Evolutionary Microbiology  
Chitinilyticum litopenaei sp. nov., isolated from a freshwater shrimp pond, and emended description of the genus Chitinilyticum Strain c1 T , originally isolated from surface water of a freshwater pond located in Pingtung (southern Taiwan) used for culture of Pacific white shrimp (Litopenaeus vannamei), was subjected to a polyphasic taxonomic analysis. The strain exhibited strong chitinolytic activity and was able to grow under aerobic and anaerobic conditions by utilizing chitin exclusively as
more » ... the carbon, nitrogen and energy source. Phylogenetic analysis of the 16S rRNA gene sequence revealed a clear affiliation to the Betaproteobacteria, with the closest relatives being Chitinilyticum aquatile C14 T and Chitinibacter tainanensis S1 T , respectively showing 96.7 and 93.6 % 16S rRNA gene sequence similarity. The predominant fatty acids detected in cells of strain c1 T were C 16 : 0 , C 18 : 1 v7c and summed feature 3 (C 16 : 1 v7c and/or iso-C 15 : 0 2-OH). The G+C content of the genomic DNA was 62.2±1.0 mol%. On the basis of phylogenetic analysis, DNA-DNA hybridization data, physiological and biochemical characteristics and fatty acid compositions, the organism was shown to belong to the genus Chitinilyticum whilst representing a novel species within this genus, for which we propose the name Chitinilyticum litopenaei sp. nov. (type strain c1 T 5DSM 21440 T 5BCRC 17609 T ). Abbreviation: GlcNAc, N-acetyl-b-D-glucosamine.
doi:10.1099/ijs.0.005090-0 pmid:19620355 fatcat:szm2qaytbrg7tgfhegxeaixa3y