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Development and evaluation of loop-mediated isothermal amplification assay for rapid detection of Capripoxvirus
2015
Veterinary World
Aim: The present study was undertaken to develop a nucleic acid-based diagnostic assay loop-mediated isothermal amplification assay (LAMP) targeting highly conserved genomic regions of Capripoxvirus (CaPVs) and its comparative evaluation with real-time polymerase chain reaction (PCR). Material and Methods: Lyophilized vaccine strain of sheeppox virus (SPPV) was used for optimization of LAMP assay. The LAMP assay was designed using envelope immunogenic protein (P32) coding gene targeting highly
doi:10.14202/vetworld.2015.1286-1292
pmid:27047031
pmcid:PMC4774739
fatcat:wywn3vy3qjaa3carm2h7n7rvcq