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Purification and characterization of transcription factors
2013
Mass spectrometry reviews (Print)
Transcription factors (TFs) are essential for the expression of all proteins, including those involved in human health and disease. However, TFs are resistant to proteomic characterization because they are frequently masked by more abundant proteins due to the limited dynamic range of capillary liquid chromatography-tandem mass spectrometry and protein database searching. Purification methods, particularly strategies that exploit the high affinity of TFs for DNA response elements on gene
doi:10.1002/mas.21369
pmid:23832591
pmcid:PMC3758410
fatcat:t3wsugpbgfco7jl5gseaqg2isi