Lignocellulolytic enzymes are extracellular biocatalysts secreted by filamentous fungi and are involved in the breakdown of recalcitrant lignocellulose plant materials into useful products necessary for fungal growth and development. Even though several studies on filamentous fungi have reported the impact of different substrates on lignocellulolyticenzymes, there is limited information on how mushroom supplements affect secretion of the enzymes, growth, andyield of Pleurotus ostreatus when

more »

... g the alkaline treatment method. In this study, we investigated the influence of cornmeal (T1) and coffee grounds (T2) supplements on lignocellulosic enzymes at different growth stages andthe production of P. ostreatus. We found that lignolytic enzyme activity was significantly higher in the control (CK)and T2 during mycelial stages, while CK had the lowest hydrolytic enzyme activity during primordia and fruiting.Unlike T1 which had the best biological efficiency, T2 exhibited significantly higher levels of lignolytic enzymesduring mycelial stage, whereas CMCase and xylanase activities were higher in supplemented treatments than in the control during primordia and fruiting. Taken together our results demonstrated that cornmeal and coffee groundsupplements reduce mycelium growth rate, enhance the production of hydrolytic enzymes during fruiting, andremarkably increase the yield and protein content of P. ostreatus.