Laeverin/aminopeptidase Q induces trophoblast invasion during human early placentation

A. Horie, H. Fujiwara, Y. Sato, K. Suginami, H. Matsumoto, M. Maruyama, I. Konishi, A. Hattori
2012 Human Reproduction  
background: In primate placenta, extravillous trophoblast (EVT) invades maternal tissue in temporally-and spatially-regulated fashions. We previously identified a novel placenta-specific cell-surface aminopeptidase, laeverin/aminopeptidase Q, which is expressed on EVT-lineage cells in the fetal membrane. Laeverin possesses a peptide-binding site that is evolutionally unique to primates, suggesting possible involvement of laeverin in a primate-specific phenomenon during placentation. Thus, this
more » ... tudy was designed to elucidate the molecular characteristics and physiological roles of laeverin in human EVT. methods: Placental tissues of various developmental stages were subjected to immunostaining and western blotting. Effects of siRNA and a soluble form of recombinant laeverin on EVT cells isolated from primary villous explant cultures were examined using Matrigel invasion assays and cell proliferation assays. results: Laeverin was specifically immunolocalized to HLA-G-positive EVT in placentas from early and term pregnancy. In primary villous explant cultures, laeverin expression was induced on the cell surface of the outgrowing EVT. In western blotting, laeverin protein was detected as two distinct bands at 130 and 160 kDa along with a broad band ranging from 200 to 270 kDa. De-glycosylation treatment showed that these native laeverin isotypes are N-linked glycoproteins sharing a common 115-kDa core protein. In invasion assays, the reduction of laeverin expression by siRNA suppressed migration of the isolated EVT, while the soluble form of recombinant laeverin enhanced its migration. conclusions: Laeverin is a specific cell-surface marker for human EVT and plays a regulatory role in EVT migration.
doi:10.1093/humrep/des068 pmid:22402206 fatcat:bm2xleqedbgznmrhnzcg5m7kqe