Integrated optical device for Structured Illumination Microscopy [article]

Matteo Calvarese, Petra Paiè, Alessia Candeo, Gianmaria Calisesi, Francesco Ceccarelli, Gianluca Valentini, Roberto Osellame, Hai Gong, Mark A. Neil, Francesca Bragheri, Andrea Bassi
2022 bioRxiv   pre-print
AbstractStructured Illumination Microscopy (SIM) is a key technology for high resolution and super-resolution imaging of biological cells and molecules. The spread of portable and easy-to-align SIM systems requires the development of novel methods to generate a light pattern and to shift it across the field of view of the microscope. Here we show a miniaturized chip that incorporates optical waveguides, splitters, and phase shifters, to generate a 2D structured illumination pattern suitable for
more » ... SIM microscopy. The chip creates three point-sources, coherent and controlled in phase, without the need for further alignment. Placed in the pupil of a microscope's objective, the three sources generate a hexagonal illumination pattern on the sample, which is spatially translated thanks to thermal phase shifters. We validate and use the chip, upgrading a commercial inverted fluorescence microscope to a SIM setup and we image biological sample slides, extending the resolution of the microscope.
doi:10.1101/2022.04.22.489094 fatcat:wklpqktpandnxm5uorweu57gpa