MOESM5 of Histone peptide microarray screen of chromo and Tudor domains defines new histone lysine methylation interactions

Erin Shanle, Stephen Shinsky, Joseph Bridgers, Narkhyun Bae, Cari Sagum, Krzysztof Krajewski, Scott Rothbart, Mark Bedford, Brian Strahl
2017 Figshare  
Additional file 5: Figure S3. CHD1 chromodomain histone peptide microarray. A) Representative array images of CHD1 chromodomain showing peptide binding indicated in red (right panel). The peptide tracer is shown in green (left panel). Positive antibody controls are outlined in white. B) Scatter plot of the relative binding of CHD1 chromodomain from two independent peptide arrays. All modified and unmodified H4 (1â 23) peptides are shown in blue, and H3K4me2/3-containing peptides are shown in
more » ... des are shown in red. All other peptides are shown in black. C) Relative binding to the indicated histone peptides from two representative arrays. Data were normalized to the most intense binding and the average and standard deviation of triplicate spots is shown.
doi:10.6084/m9.figshare.c.3716059_d5.v1 fatcat:ttwydilqxbgsvceijnqt32sia4