The Influence of Various Toxic Effects on the Cornea and Lens Induced by Carbon Tetrachloride in Mice and the Antioxidant Effects of Spirulina platensis

Eman M. Aly, Haiam M. Aboul-Ela, Mervat A. Aly
2016 Indian Journal of Science and Technology  
Objective: The present study was directed to examine the toxicity of carbon tetrachloride (CCl 4 ) in ocular tissues of Swiss Albino mice and the strong antioxidant beneficial effects of Spirulina platensis. Methods/Analysis: Na + -K + ATPase measurement was done on the lens membrane. Soluble lens proteins were extracted and the following techniques were made: evaluation of total soluble protein and gel filtration chromatography. Likewise, the corneal protein secondary structure was evaluated
more » ... ure was evaluated by Fourier transform infrared spectroscopy (FTIR). For comparison between multiple groups, analysis of variance was used with significance level set at P < 0.001. Finding: The results demonstrated a decrease in total soluble lens protein and Na + -K + ATPase activity, increase in molecular weight of all lens protein fractions and there are different structural and conformational changes in cornea after injection of CCl 4 . These changes were reduced in pretreated mice with Spirulina. Novelty/Improvement: Our study clearly concluded that Spirulina platensis shows a protective effect on the lens and cornea through its antioxidant activity on CCl 4 -induced toxicity in mice.
doi:10.17485/ijst/2016/v9i35/90894 fatcat:avszpr3kazgylpkdsczrvx2kgq