Role of a small RNA pol II subunit in TATA to transcription start site spacing

Elizabeth M. Furter-Graves, Benjamin D. Hall, Rolf Furter
1994 Nucleic Acids Research  
The yeast shi mutation affects the spacing between the TATA promoter element and transcription initiation sites; for the H2B and ADH1 genes, a series of start sites located -50 -80 bp downstream of TATA is used in addition to the wild-type initiation sites located at around 100 bp from TATA (1). Here, the yeast SHI wildtype gene has been isolated by complementation and shown to be identical to RPB9, the gene encoding a small subunit of RNA polymerase 11. A point mutation in the shi gene,
more » ... g a cysteine residue in a putative zinc ribbon motif into a phenylalanine residue, was demonstrated to permit the observed usage of upstream initiation sites. Deletion of the non-essential SHI gene also results in usage of upstream initiation sites and causes conditional growth defects. Plasmids Plasmid pCEN-SHI/1.5 was constructed by inserting the 1.5 kb PstI -HincH fragment encoding the putative SHI gene (see Fig.
doi:10.1093/nar/22.23.4932 pmid:7800482 pmcid:PMC523758 fatcat:tuqitlvmqfgh5oc5yb5dalvvum