Prospective methods for human papillomavirus detection by fluorescent in situ hybridization and laser excitation

G. LIZARD, C. SOUCHIER, Y. CHARDONNET
1994 Journal de Physique IV : Proceedings  
As some human papillomaviruses (HPV) have an oncogenic potential, it is of interest to develop cytological methods which allow the detection of low copy numbers of HPV DNA. The human uterine carcinoma cell lines CaSki, SiHa and HeLa were used since they contain different copy numbers of HPV DNA per cell: 500-600 copies of HPV16, 1-2 copies of HPV 16 and 20-50 copies of HPV18, respectively. HPV DNA was revealed by fluorescent in situ hybridization (FISH) with biotinylated
doi:10.1051/jp4:1994459 fatcat:v36j2lthbvaqholmwtxrvwjqwi