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In Vitro Excystation and Cryoreservation of Ovine and Caprine Sarcocystis species
The Journal of protozoology research
This paper describes a standard method for in vitro excystation of sporocysts of S.capracanis, S.hircicanis, S.ovicanis and S.arieticanis. A standard pretreatment was performed by 20 minutes preincubation of sporocysts in aqueous sodium hypochlorite (NaOCl) solution of 6% or 8% at room temperature. Then, sporocysts were washed 5 times in distilled water and were incubated for one hour at 39 O C in excystation fluid consisting of RPMI 1640 medium, 10% PCS and 15% bovine bile. Additionaldoi:10.32268/jprotozoolres.1.0_13 fatcat:ojkqmtejznetblk577ncwbec5y