Cryo-SEM Methodology of Arabidopsis thaliana Stem Using High-Pressure Freezing
고압동결고정을 이용한 애기장대 줄기의 cryo-SEM 분석법

Yun-Joung Choi, Kyung-Hwan Lee, A-Reum Je, Hee-Su Chae, Ji-Hoon Jang, Eun-Ji Lee, Hee-Seok Kweon
2012 Korean Journal of Microscopy  
The scanning electron microscopy is an ideal technique for examining plant surface at high resolution. Most hydrate samples, however, must be fix and dehydrate for observation in the scanning electron microscope. Because the microscopes operate under high vacuum, most specimens, especially biological samples, cannot withstand water removal by the vacuum system without morphological distortion. Cryo-techniques can observe in their original morphology and structure without various artifacts from
more » ... ous artifacts from conventional sample preparation. Rapid cooling is the method of choice for preparing plant samples for scanning electron microscopy in a defined physiological state. As one of cryo-technique, highpressure freezing allows for fixation of native non-pretreated samples up to 200 μm thick and 2 mm wide with minimal or no ice crystal damage for the freezing procedure. In this study, we could design to optimize structural preservation and imaging by comparing cryo-SEM and convention SEM preparation, and observe a fine, well preserved Arabidopsis stem's inner ultrastructure using HPF and cryo-SEM. These results would suggest a useful method of cryo-preparation and cryo-SEM for plant tissues, especially intratubule and vacuole rich structure. (최윤정, 이경환, 제아름, 채희수, 장지훈, 이은지, 권희석: 고압동결고정을 이용한 애기장대 줄기 의 cryo-SEM 분석법)
doi:10.9729/am.2012.42.2.111 fatcat:rdcm6xh3gzbrzbfb7d37w4uk2a