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Characterization and translation of methylated and unmethylated vesicular stomatitis virus mRNA synthesized in vitro by ribonucleoprotein particles from vesicular stomatitis virus-infected L cells
1976
Journal of Virology
Preserved Fulltext
Ribonucleoprotein particles isolated from extracts of vesicular stomatitis virus (VSV)-infected L cells synthesized in vitro four classes of polyadenylated RNA sedimenting at 29S, 19S, 17S, and 13S. When synthesized in vitro in the presence of the methyl donor S-adenosyl methionine, these RNA species contained the following 5'-terminal structures: (i) m7G5 ppp5'AmpAp (70%); (ii) M 7G 5'ppp5 AmpAmpNp (20%); and (iii) pppAp (10%). In the presence of the methylation inhibitor
doi:10.1128/jvi.17.2.477-491.1976
fatcat:o6k6hi5fazc5far2v6bhj7aysa