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Origin-Dependent Neural Cell Identities in Differentiated Human iPSCs In Vitro and after Transplantation into the Mouse Brain

Gunnar Hargus, Marc Ehrlich, Marcos J. Araúzo-Bravo, Kathrin Hemmer, Anna-Lena Hallmann, Peter Reinhardt, Kee-Pyo Kim, Kenjiro Adachi, Simeon Santourlidis, Foued Ghanjati, Mareike Fauser, Christiana Ossig (+7 others)
2014 Cell Reports  

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Graphical Abstract Highlights Human iPSCs demonstrate origin-dependent expression profiles after neural induction NPCs from neuroectoderm-derived iPSCs share reprogramming memory with fetal NSCs Origin-dependent neural cell identities are retained after transplantation This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). SUMMARY The differentiation capability of induced pluripotent stem cells (iPSCs) toward certain cell types for
more » ... se modeling and drug screening assays might be influenced by their somatic cell of origin. Here, we have compared the neural induction of human iPSCs generated from fetal neural stem cells (fNSCs), dermal fibroblasts, or cord blood CD34 + hematopoietic progenitor cells. Neural progenitor cells (NPCs) and neurons could be generated at similar efficiencies from all iPSCs. Transcriptomics analysis of the whole genome and of neural genes revealed a separation of neuroectoderm-derived iPSC-NPCs from mesoderm-derived iPSC-NPCs. Furthermore, we found genes that were similarly expressed in fNSCs and neuroectoderm, but not in mesodermderived iPSC-NPCs. Notably, these neural signatures were retained after transplantation into the cortex of mice and paralleled with increased survival of neuroectoderm-derived cells in vivo. These results indicate distinct origin-dependent neural cell identities in differentiated human iPSCs both in vitro and in vivo.
doi:10.1016/j.celrep.2014.08.014 pmid:25220454 fatcat:m4malts6xbc63l4zxtoz6vtu2q
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Gunnar Hargus, Marc Ehrlich, Marcos J. Araúzo-Bravo, Kathrin Hemmer, Anna-Lena Hallmann, Peter Reinhardt, Kee-Pyo Kim, Kenjiro Adachi, Simeon Santourlidis, Foued Ghanjati, Mareike Fauser, Christiana Ossig, Alexander Storch, Jeong Beom Kim, Jens C. Schwamborn, Jared Sterneckert, Hans R. Schöler, Tanja Kuhlmann, Holm Zaehres. "Origin-Dependent Neural Cell Identities in Differentiated Human iPSCs In Vitro and after Transplantation into the Mouse Brain." Cell Reports 8.6 (2014) 1697-1703