Workshop

2013 Experimental animals  
Classic histological technique using the paraffin sections followed by hematoxylin and eosin staining is a basic method to detect morphological anomalies of mutants or to compare morphological features of model animals. Though this method is well established and widely performed everywhere, there are following weak points. Preparation of the excellent sections needs dexterous manipulation, and is time-consuming. Reconstruction of the 3D images from the 2D section information is arduous task. In
more » ... medical fields, multi-detector row computed tomography (MDCT) is now commonly used for diagnosis, and becoming a powerful tool for detection and classification of human symptomatic state. X-ray micro-computed tomography (micro-CT) for model animals is identical in its basic principles to MDCT. Micro-CT easily generates 2D slice images at any position and angle, and thereby reconstructs 3D image. It provides excellent images of bone and adipose tissues, because these samples have high contrast Hounsfield unit. Adversely, it has not provided good images of other non-mineralized soft tissues. In this workshop, we would like to introduce an improved method to obtain high resolution of 2D and 3D images of soft tissues in mouse embryos using the micro-CT with inorganic iodine as a contrast reagent. We also argue about the possibility of this method for future high-throughput morphometrics from various aspects, e.g., degree of resolution, simplicity and costs.
doi:10.1538/expanim.62.s25 fatcat:snglwjul5femziivxnbljd4ele