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Classic histological technique using the paraffin sections followed by hematoxylin and eosin staining is a basic method to detect morphological anomalies of mutants or to compare morphological features of model animals. Though this method is well established and widely performed everywhere, there are following weak points. Preparation of the excellent sections needs dexterous manipulation, and is time-consuming. Reconstruction of the 3D images from the 2D section information is arduous task. Indoi:10.1538/expanim.62.s25 fatcat:snglwjul5femziivxnbljd4ele