Chemical component and acute toxicity study of Erythrococca anomala (Euphorbiaceae) MATERiAl And METhodS Plant material QR Code for mobile Open Access Literati Research Article

Miezan Bilé, Aka Patrice, Aboua Timothée, Yapi Houphouët Félix, Gbassi Gildas, Assi Yapo, Jean
2016 Asian Journal of Biomedical and Pharmaceutical Sciences   unpublished
Erythrococca anomala (Juss. ex. Poir) Pain (Euphorbiaceae) is a medicinal plant widely used in sub-saharan Africa. It is popular against certain diseases such as malaria, arthritis, rheumatism and toothache. However, there are no data on its phytochemical and biological profile , hence the importance of this study is to search for chemical groups of this plant and to determine the toxicological parameters that justify its use in the traditional medicine. Standard characterization methods and
more » ... tion methods and thin layer chromatography (TLC) were used for the phytochemical screening. The acute toxicity study of Erythrococca anomala was performed according to the guideline OECD 423 using Wistar rats. Phytochemical screening revealed the presence of polyphenols, alkaloids, catechol tannins, saponins, leucoanthocyanins, fla-vonoids, polyterpenes and sterols which could justify the biological and pharmacological properties of this herb. The acute toxicity study of the extracts, administered intraperito-neally in Wistar rats, was used to determine the 50% lethal dose (LD 50) value to be 741.31 mg/kg of body weight (BW), 100% lethal dose (LD 100) corresponding to 2000 mg/kg BW and maximum tolerated dose (MTD) to 700 mg/kg BW. These toxicological data allow us to qualify Erythrococca anomala at very low toxic hence its importance in the traditional use against malaria and multifaceted pain. ABSTRACT : Erythrococca anomala (Juss. ex Poir.) Prain (Euphorbiace-ae), thorny shrub and dioecious, up to 3m high, is a nutritional and medicinal plant in sub-Saharan Africa. In Côte d'Ivoire, leaf powder, alone or mixed with that of Psycho-tria peduncularis is used by friction against malaria and meningitis. 1,2 In Cameroon, the decoction or maceration of leaves, laxative and purgative, is taken to expel tape-worms or treat dental pain while in Nigeria the bark is used against arthritis and rheumatism. The fruit pulp and root bark are also used as a tonic against asthenia. 3 E. anomala is used successfully in Africa on the basis of experiences of populations. In order to rationalize its use, we have initiated this study to search for the major chemical groups and assess to the acute toxicity of aqueous and hydroethanolic 70% extracts of Erythrococca anomala. Both lethal doses 50% (LD 50) and 100% (DL 100) and the maximum tolerated dose (MTD) are also determined.