Buffers for Chloroplast Isolation from Diatoms v1 [dataset]

P Dreux, Bethany D
2015 protocols.io  
1 1 1 Guide for reagent/buffer preparation for protocol to separate chloroplasts from diatom cells using ammonium fluoride to permeate the silica frustrule and a percoll gradient to separate the plastid from other cellular components. Buffers and reagents can be mixed up ahead of time and stored cold (refrigerated). Percoll gradients (layering of 40% and 80% percoll solutions) should be done the same day of the extraction shortly before use.
doi:10.17504/protocols.io.ea2bage fatcat:aeev5pe5orb7hhwlnsysbuxgmy