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Buffers for Chloroplast Isolation from Diatoms v1
[dataset]
2015
protocols.io
Preserved Fulltext
1 1 1 Guide for reagent/buffer preparation for protocol to separate chloroplasts from diatom cells using ammonium fluoride to permeate the silica frustrule and a percoll gradient to separate the plastid from other cellular components. Buffers and reagents can be mixed up ahead of time and stored cold (refrigerated). Percoll gradients (layering of 40% and 80% percoll solutions) should be done the same day of the extraction shortly before use.
doi:10.17504/protocols.io.ea2bage
fatcat:aeev5pe5orb7hhwlnsysbuxgmy