Characterization of a bovine herpesvirus 4 immediate-early RNA encoding a homolog of the Epstein-Barr virus R transactivator

V L van Santen
1993 Journal of Virology  
Immediate-early (IE) RNA 2, the less abundant of two bovine herpesvirus 4 (BHV-4) RNAs detected in Madin-Darby bovine kidney cells infected in the presence of cycloheximide, is a 1.8-kb cytoplasmic polyadenylated RNA transcribed from the 8.3-kb HindUI fragment F. The structure of IE RNA 2 has been determined by Si nuclease and exonuclease VII mapping, primer extension analysis, and sequencing of a partial cDNA. IE RNA 2 consists of a short, approximately 60-nucleotide 5' exon spliced to a
more » ... spliced to a 1.8-kb 3' exon. DNA sequence analysis revealed an open reading frame encoding 551 amino acids with sequence homology to the Epstein-Barr virus (EBV) R transactivator and its homolog in herpesvirus saimiri, HVS.R. IE 2 and EIVS.R show higher homology to each other than to the EBV R transactivator. The homology is highest in the approximately 320 amino-terminal amino acids. All three proteins have acidic carboxyl termini but have little amino acid sequence homology in this region. In transient expression cotransfection assays, IE 2 activated expression from the BHV-4 early promoter-regulatory region of the major DNA-binding protein homolog over the BHV-4 E gene encoding the BHV-4 homolog to the herpes simplex virus type 1 (HSV-1) major DNA-binding protein. However, BHV-4 IE 2 protein does not transacti- Vol. 67, No. 2 on May 4, 2020 by guest
doi:10.1128/jvi.67.2.773-784.1993 fatcat:qczcb4xgi5a65ialal42e7kcne