Comparative analysis of cat bone marrow and adipose tissue cell cultures

A Mazurkevych, M Malyuk, V Kovpak, O Kovpak, Y Kharkevych, A Jakubczak, M Gryzinska
2018 Polish journal of veterinary sciences  
Cell culture transplantation is very promising in the treatment of various diseases. Cells obtained from a number of sources have been analysed to provide a basis for further studies in the area of regenerative medicine. The objective of the study was to compare morphological and phenotypic changes in cat adipose tissue and bone marrow cell cultures from the first to fifth passages. Adipose tissue and bone marrow were used to obtain cell cultures (coming from 3 cats) using standard methods with
more » ... own modification. Phenotype changes were monitored by CD-marker identification and CD pan-keratin. The cytogenetic analysis was performed on 50 metaphase plates of cell cultures from the first to fifth passage. Cytogenetic assays showed that the adipose tissue cell culture (ATCC) at all passages was more stable than the bone marrow cell culture (BMCC).
doi:10.24425/124289 pmid:30468354 fatcat:5wi2kyrbunbnbk2rifocvbrtva