Objective To explore the chemo-sensitizing effect and inhibitory effect of Ganoderma lucidum polysaccharide (GLP) on tumor angiogenesis of bladder cancer. Methods The effect of GLP combined with cisplatin on the in vitro proliferation of human bladder cancer cell lines T24 was determined by MTS assay. The interaction between the two drugs was evaluated using the Chou-Talalay method. A T24 (bladder cancer)-bearing nude mouse model was established, and then the therapeutic effect of GLP combined

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... ith cisplatin was evaluated. The microvessel density (MVD) and the expressions of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in the tumor tissue were determined by immunohistochemical staining, and the expression levels of VEGF and bFGF in tumor tissue were determined by real-time fluorescence quantitative PCR and Western blotting. Results GLP combined with cisplatin markedly inhibited T24 cells proliferation in vitro showing a synergistic effect (CI<1). Immunohistochemical staining showed that GLP could enhance the inhibitory effect of cisplatin on the tumor growth in the tumorbearing nude mice (P<0.05), and it could also inhibit the angiogenesis and expressions of VEGF and bFGF in the tumor tissue. The real-time fluorescence quantitative PCR and Western blotting showed that the expression levels of VEGF and bFGF in tumor tissue significantly decreased in the tumor-bearing nude mice after being treated with GLP combined with cisplatin as compared with those treated by cisplatin alone. Conclusions GLP combined with cisplatin could inhibit the proliferation of T24 cells in vitro synergically. GLP could enhance the inhibitory effect of cisplatin on tumor growth and angiogenesis in T24 tumor-bearing nude mice, and the mechanism may be related to the down-regulation of VEGF and bFGF expressions. DOI: 10.11855/j.issn.0577-7402.2014.06.09