Studies on the gynogenesis in hirame Paralichthys olivaceus. II Induction of gynogenetic diploid in hirame Paralichthys olivaceus
ヒラメの雌性発生に関する研究 II 紫外線によるヒラメの雌性発生2倍体の誘起条件

Kazuo Tabata, Shigeaki Gorie, Kazuhiko Nakamura
1986 Nippon Suisan Gakkaishi  
Induction of gynogenetic diploid in hirame Paralichthys olivaceus Kazuo Tabata,*2 Shigeaki Gorie,*2 and Kazuhiko Nakamura*2 The following results were obtained from the experiments toind optimum conditions for induction of gynogenetic diploids in hirame Paralichthys olivaceus. The Ringer solutions for Pleuronectes and freshwater fish seem to be adequate as the diluting solution for milt of hirame because spermatozoa aren't motile in either Ringer solution and resisted for at least 3 hours when
more » ... hey were stored at 3℃. Keeping temperature constant during irradiation is required because the intensity of UV depended on the temperature. The hatching rate of eggs inseminated with milt irradiated by UV rays was almost 0% at the dosage of 110-230 erg/mn2, but it recovered when milt was irradiated with more than 660 erg/mm2 and all frys showed to haploid syndrome(n=24). Gynogenetic diploids of hirame were induced by cold shock treatment-put the fertilised eggs in sea water of0℃ for 45-60 min from 2-5 min after insemination with milt irradiated by UV rays at the dosage of 660-1,380 erg/mm2. Heat shocks were not effective for induction of gynogenetic diPloid.
doi:10.2331/suisan.52.1901 fatcat:e4lq54lpgbchnfkjt2byco4zwe