Flow Injection Analysis of Immunoglobulin G Using Surface Plasmon Resonance Sensor
表面プラズモン共鳴センサを利用した免疫グロブリンGのフローインジェクション分析

Ryoichi Ishimatsu, Ruifen Zhang, Yuko Kirino, Koji Nakano, Toshihiko Imato
2014 FIA研究懇談会会誌  
Direct detection of immunoglobulin G (IgG) by using a surface plasmon resonance (SPR) sensor through an antigen-antibody interaction was described. An anti-IgG antibody was chemically immobilized on a sensor chip through a self-assembled monolayer (SAM) in order to enhance the recognition of an IgG antigen. By assuming the Langmuir adsorption isotherm, maximum amount of adsorbed anti-IgG antibody on the sensor chip and an apparent biding constant of the IgG antigen to anti-IgG antibody were
more » ... mated. The amount of the IgG antigen bound to the anti-IgG antibody, which was immobilized onto the sensor chip through the SAM was three times higher compared with the amount of the IgG antigen bound to the anti-IgG antibody, which was immobilized directly to the sensor chip through physical adsorption.
doi:10.24688/jfia.31.2_111 fatcat:6mwzrsik7jfc7hbdb6tkdmro6e