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Flow Injection Analysis of Immunoglobulin G Using Surface Plasmon Resonance Sensor
表面プラズモン共鳴センサを利用した免疫グロブリンGのフローインジェクション分析
2014
FIA研究懇談会会誌
表面プラズモン共鳴センサを利用した免疫グロブリンGのフローインジェクション分析
Direct detection of immunoglobulin G (IgG) by using a surface plasmon resonance (SPR) sensor through an antigen-antibody interaction was described. An anti-IgG antibody was chemically immobilized on a sensor chip through a self-assembled monolayer (SAM) in order to enhance the recognition of an IgG antigen. By assuming the Langmuir adsorption isotherm, maximum amount of adsorbed anti-IgG antibody on the sensor chip and an apparent biding constant of the IgG antigen to anti-IgG antibody were
doi:10.24688/jfia.31.2_111
fatcat:6mwzrsik7jfc7hbdb6tkdmro6e