Multilocus Sequence Types and Virulence Determinants of Hypermucoviscosity-Positive Klebsiella pneumoniae Isolated from Community-Acquired Infection Cases in Harbin, North China

Jizi Zhao, Jie Chen, Ming Zhao, Xiaohong Qiu, Xiaobei Chen, Wenli Zhang, Rui Sun, James Opiyo Ogutu, Fengmin Zhang
2016 Japanese journal of infectious diseases (Print)  
We investigated the molecular epidemiologic characteristics and virulence of hypermucoviscosity-positive Klebsiella pneumoniae in mainland China. We detected 16 hypermucoviscositypositive strains in 65 total clinical isolates (24.62z). We found that 68.75z (11/16) of the positive strains had K2 genotype and carried the rmpA and iucA genes. Multilocus sequence typing revealed 5 sequence types (STs) : ST65 [7], ST23 [4], ST86 [3], ST412 [1], ST375 [1], whereas the remaining 4 isolates were
more » ... as other STs. The order of the median lethal dose values for the ST types was ST23 (2.19 × 10 3 CFU/mouse) < ST86 (1.70 × 10 4 CFU/mouse) < ST65 (5.05 × 10 7 CFU/mouse) < the other STs (1.90 × 10 8 CFU/mouse). In conclusion, the K2 with ST65 carrying rmpA and iucA was the most predominant among the hypermucoviscosity-positive K. pneumoniae strains obtained from communityacquired infection cases in Harbin, North China. Sequence types are a valuable tool to predict the risk of K. pneumoniae infection.
doi:10.7883/yoken.jjid.2015.321 pmid:26743146 fatcat:y4zf7peilrfk3l5za6alkzgoeq