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Dicer-independent RNA-directed DNA methylation in Arabidopsis
2015
Cell Research
RNA-directed DNA methylation (RdDM) is an important de novo DNA methylation pathway in plants. Small interfering RNAs (siRNAs) generated by Dicers from RNA polymerase IV (Pol IV) transcripts are thought to guide sequence-specific DNA methylation. To gain insight into the mechanism of RdDM, we performed whole-genome bisulfite sequencing of a collection of Arabidopsis mutants, including plants deficient in Pol IV (nrpd1) or Dicer (dcl1/2/3/4) activity. Unexpectedly, of the RdDM target loci that
doi:10.1038/cr.2015.145
pmid:26642813
pmcid:PMC4816133
fatcat:pelzcoupl5hkhdepgxwr376eru