Abstract 162: Profiling of microRNA in oral cancer cells identifying miR-10b functions in oncogenesis and up-regulates in plasma of oral cancer patients

Ya-Ching Lu, Ann-Joy Cheng
2011 Cancer Research  
Oral cancer is the 6 th leading cancer, and is still increasing with years. Since this cancer usually occurs in the middle age male, at the high peak of life responsibility, it has tremendous impact of family and society. MicorRNAs (miRNAs) are endogenous, non-coding small RNA, which consist of 21-23 nucleotides. They serve widespread functions as regulatory molecules in post-transcriptional gene silencing for the regulation of cellular bio-functions. And the dysregulation of miRNAs has been
more » ... miRNAs has been implicated in malignancy. Agilent system was used to perform miRNA profiling in 6 cancer cell lines and 5 lines of normal keratinocytes for identification of differently expressed miRNAs. There were 23 miRNAs selected as candidates which were shown more than 2-fold change with the FDR<0.1. Among these miRNAs, 19 of them were elevated and 4 were decreased in oral cancer cell lines. miR-10b was validated by qPCR analysis showed significantly up-regulated in cancer cells. Furthermore, the biological function of miR-10b was investigated after alteration of miR-10b expression. Reduction of miR-10b by antisense oligonucleotide further resulted in retard of cell migratory and invasive ability, however, no significant alterations on cell growth or chemo/radio-sensitivity. In analyzing miR-10b expression in 24 normal and 29 plasma samples from oral cancer patients, miR-10b was up-regulated 3.3 fold in the cancer patients (P<0.0001). Taken together, miR-10b plays a role in the carcinogenesis through promoting cancer cell migratory and invasive function, and plasma miR-10b may serve as a biomarker for oral cancer patients. . (2005) Univariate and multivariate analysis of prognostic significance of betel quid chewing in squamous cell carcinoma of buccal mucosa in Taiwan. J Surg Oncol, 91, 41-7. 4. . (2008). Analysis of risk factors of predictive local tumor control in oral cavity cancer. Ann Surg Oncol, 15, 915-22. 7. Wienholds E, Plasterk RH. MicroRNA function in animal development. FEBS Lett 579: 5911-5922, 2005. 8. Garzon R, Fabbri M, Cimmino A, Calin GA, Croce CM. MicroRNA expression and function in cancer. Trends in Mol Med 12: 581-587, 2007. 9. Berezikov E, et al. Philogenetic shadowing and computational identification of human micro RNAs. Cell 120: 21-24, 2005.
doi:10.1158/1538-7445.am2011-162 fatcat:qs4wtr2w3vcblgpuzbec5nm3si