Hydrolysis of lactose with -D-galactosidase

Vesna Stehlik-Tomas, Slobodan Grba, Damir Stanzer, Vlatka Gulan-Zetić
2001 Mljekarstvo  
The conditions of lactose hydrolysis with enzyme preparation of D-galactosidase were investigated. The aim of this work was to considered the use of whey in fermentative processes with yeast Saccharomyces cerevisiae. Enzymatic hydrolysis was conducted at different temperatures, with different lactose concentrations in medium and different concentrations of added enzyme. The results show that optimal temperature for hydrolysis was 40°C. The optimal amount of enzyme preparation was 2 gL-1 in lactose medium with 5-10 % lactose.
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