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Vasoactive Intestinal Polypeptide-like Immunoreactivity in a Human Neuroblastoma Cell Line and the Coexistence of Other Neuropentide Immunoreactivity in the Cell Line

NOBORU YANAIHARA, SHIGERU KOBAYASHI, HARUKO SATO, CHIZUKO YANAIHARA, MASANORI SAKAGAMI, NAOKI SAKURA, TADASHI HASHIMOTO, SHINOBU ISHIKAWA, TOSHIHIKO IWANAGA, TSUNEO FUJITA
1980 Endocrinologia Japonica  

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Synopsis The presence of VIP-like immunoreactivity in human neuroblastoma cell line NB-1 was demonstrated by radioimmunoassay of the crude cell extract and by immunocytochemical staining of the cells. Gelfiitration profiles of VIP-like immunoreactivity in the cell extract measured by radioimmunoassays using three different region-specific antisera revealed that the immunoreactivity consists of a major molecular form corresponding to porcine VIP having 28 amino acid residues with at least two
more » ... itional minor forms larger and smaller than the VIP. In addition to VIP-like immunoreactivity, the cell extract was shown to containsubstance P-, neurotensin-and somatostatin-like immunoreactivitiy as well.
doi:10.1507/endocrj1954.27.supplement_37 pmid:6164547 fatcat:faa2lvhm2zeexfg2zeo4zsvoni
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NOBORU YANAIHARA, SHIGERU KOBAYASHI, HARUKO SATO, CHIZUKO YANAIHARA, MASANORI SAKAGAMI, NAOKI SAKURA, TADASHI HASHIMOTO, SHINOBU ISHIKAWA, TOSHIHIKO IWANAGA, TSUNEO FUJITA. "Vasoactive Intestinal Polypeptide-like Immunoreactivity in a Human Neuroblastoma Cell Line and the Coexistence of Other Neuropentide Immunoreactivity in the Cell Line." Endocrinologia Japonica 27.Supplement (1980) 37-42