Intensity-dependent gene expression after aerobic exercise in endurance-trained skeletal muscle

Daniil V Popov, Pavel A Makhnovskii, Nadia S Kurochkina, Evgeny A Lysenko, Tatiana F Vepkhvadze, Olga L Vinogradova
2018 Biology of Sport  
We investigated acute exercise-induced gene expression in skeletal muscle adapted to aerobic training. Vastus lateralis muscle samples were taken in ten endurance-trained males prior to, and just after, 4 h, and 8 h after acute cycling sessions with different intensities, 70% and 50% · VO 2max . High-throughput RNA sequencing was applied in samples from two subjects to evaluate differentially expressed genes after intensive exercise (70% · VO 2max ), and then the changes in expression for
more » ... xpression for selected genes were validated by quantitative PCR (qPCR). To define exercise-induced genes, we compared gene expression after acute exercise with different intensities, 70% and 50% · VO 2max , by qPCR. The transcriptome is dynamically changed during the first hours of recovery after intensive exercise (70% · VO 2max ). A computational approach revealed that the changes might be related to up-and down-regulation of the activity of transcription activators and repressors, respectively. The exercise increased expression of many genes encoding protein kinases, while genes encoding transcriptional regulators were both up-and down-regulated. Evaluation of the gene expression after exercise with different intensities revealed that some genes changed expression in an intensity-dependent manner, but others did not: the majority of genes encoding protein kinases, oxidative phosphorylation and activator protein (AP)-1-related genes significantly correlated with markers of exercise stress (power, blood lactate during exercise and postexercise blood cortisol), while transcriptional repressors and circadian-related genes did not. Some of the changes in gene expression after exercise seemingly may be modulated by circadian rhythm. CITATION: Popov DV, Makhnovskii PA, Kurochkina NS et al. Intensity-dependent gene expression after aerobic exercise in endurance-trained skeletal muscle. Biol Sport. 2018;35(3):277-289. . tigated only in one study, with a limited number of genes (220) monitored [35]. It has been shown that activation of signalling proteins and expression of genes in response to acute aerobic exercise are less pronounced (more specific) in human skeletal muscle adapted to endurance training in comparison with untrained muscle [22, 27, 35, 43]. Moreover, low substrate availability induces changes in the expression of metabolic genes in the skeletal muscle of humans during recovery from exercise [28]. Most of the transcriptome studies cited above involved untrained subjects and investigated transcriptome response in the fasted state, and only one study involved endurance-trained subjects under normal conditions -in the feeding state [21]. The first part of our study was exploratory; we sought changes in the transcriptome in endurance-trained skeletal muscle during the early (4 h) and middle (8 h) stages of recovery from acute intensive
doi:10.5114/biolsport.2018.77828 fatcat:mgoyfyxry5co3jczbtwbx6bbqu