Cloning and Characterization of a Novel Chromosomal Drug Efflux Gene in Staphylococcus aureus

Koji Narui, Norihisa Noguchi, Kazunori Wakasugi, Masanori Sasatsu
2002 Biological and Pharmaceutical Bulletin  
A novel drug efflux gene (named sepA, staphylococcal efflux pump gene) is cloned from antiseptic-resistant mutants of Staphylococcus aureus into Escherichia coli. The sepA gene conferred the reduction of susceptibility to acriflavine and the acceleration of ethidium bromide efflux from the E. coli cells. The sepA (474 bp) encoded the protein that has four predicted transmembrane segments. These results indicate that sepA gene is a multidrugresistant gene and encodes a drug efflux protein.
doi:10.1248/bpb.25.1533 pmid:12499635 fatcat:ymirsz75gbdprkch2w6xjtrmka