Role of p63 isoform balance in recurrent nasal polyps

Alessandro Terrinoni, Ramona Palombo, Consuelo Pitolli, Sabrina Caporali, Rita De Berardinis, Silvana Ciccarone, Alessia Lanzillotta, Simone Mauramati, Gerry Melino, Sergio Bernardini, Ernesto Bruno
2018 Otorhinolaryngology-Head and Neck Surgery  
Objective: Nasal polyps are, benign growths outgrowths of sinonasal tissue, affecting approximately 1-4% of the population. They have been correlated to the presence of chronic inflammation, but the molecular underlying mechanisms has not been completely defined. Defective epithelial barrier has been correlated with chronic rhinosinusitis (CRS) and nasal polyps (NPs) Moreover, a variation of ΔNp63 isoform expression has been noted in human airway epithelial cells and in the NPs epithelium. The
more » ... enign lesion in some patients can have a recurrence, and the difference between recurrent and not recurrent patients is still unclear. The aim of the study was to molecularly characterise NPs patients, distinguishing between patients presenting NP recurrence after surgical treatment respect to those in which recurrence has been not reported. Methods: In the present work we analysed a cohort of patients affected by nasal polyposis, all submitted to Functional Endoscopic Sinus Surgery (FESS). The patients have been six-monthly clinical, checked in the two years following surgery. In all patients we analysed keratin 5, and p63 isoform expression, by RTqPCR using fresh NPs tissues taken after surgery. Moreover, confocal imunnofluorescence analysis again for p63 and Keratin 5 has been performed on fixed sections. Results and conclusion: The results show high ΔNp63 expression and keratin 5, in nasal polyps of patients. The analysis of the expression level of the TAp63 isoform, shows a differential expression between patients with recurrence, respect to the not recurring. A more comprehensive marker could be considered the ΔN/TAp63 ratio. In fact, even though ΔNp63 is expressed in non-recurrent patients the ratio ΔN/TAp63 result significantly lower in these patients. This clearly indicates that the status of TA63 expression, and represented by ΔN/TAp63 ratio, could be considered a prognostic markers of low recurrence probability. Since TAp63 is also upregulated by HDAC inhibitors could be the consideration of local treatment of nasal polyps with these molecules.
doi:10.15761/ohns.1000193 fatcat:3n2iuqed6vd5rgqwtninikhre4