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The Proceedings of Mechanical Engineering Congress Japan
In this paper, non-cryopreservation method for killifish eggs was conducted. It is known that cells become low activity at low temperature, and also freezing point depresses to -20℃ under high hydrostatic pressure of 200MPa. Thus, it is expected that cells can be preserved without damage by ice crystal by applying high hydrostatic pressure. In order to verify this phenomenon, the authors developed an experimental device that can conduct in-situ observation of living cells in a high hydrostaticdoi:10.1299/jsmemecj.2016.j0260106 fatcat:vwtima6kf5hfndh7lw2rd2rxne