In this paper, non-cryopreservation method for killifish eggs was conducted. It is known that cells become low activity at low temperature, and also freezing point depresses to -20℃ under high hydrostatic pressure of 200MPa. Thus, it is expected that cells can be preserved without damage by ice crystal by applying high hydrostatic pressure. In order to verify this phenomenon, the authors developed an experimental device that can conduct in-situ observation of living cells in a high hydrostatic

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... ressure chamber. Using this device, pressuring and cooling experiments for killifish eggs were conducted. As a result, the authors found that the pressuring and cooling path affected on the survival rate of the killifish eggs. Cooling before pressuring path have a higher survival rate than pressuring before cooling path. This is because the eggs become low activity in low temperature, and the damage caused by pressuring become small.