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Whole-cell cryo-electron tomography of cultured and primary eukaryotic cells on micropatterned TEM grids
Whole-cell cryo-electron tomography (cryo-ET) is a powerful technique that can provide nanometer-level resolution of biological structures within the cellular context and in a near-native frozen-hydrated state. It remains a challenge to culture or adhere cells on TEM grids in a manner that is suitable for tomography while preserving the physiological state of the cells. Here, we demonstrate the versatility of micropatterning to direct and promote growth of both cultured and primary eukaryoticdoi:10.1101/2021.06.06.447251 fatcat:cm6gb4z3c5czhe7uawjiq6wsya