THE APPLICATION OF CENTRAL COMPOSITE DESIGN FOR OPTIMIZATION OF KETOPROFEN NIOSOMES
The objective of the present study was to investigate the effect of important formulation variables on drug entrapment in and drug release from niosome formulations of ketoprofen to obtain an optimized formula of Ketoprofen niosomes using central composite design. Contour and response surface plots were depicted based on the equation given by the model of the form Y=b0+b1X1+b2X2+b3X3+b1 2 X11+b2 2 X22+b3 2 X33+b12X1X2+b13X1X3+b23X2X3+b123X1X2X3 where Y is the measured response associated with
... e associated with each factor level combination. Niosomes were prepared by a lipid hydration method using central composite design with three different variables include; Surfactant cholesterol ratio (X1), HLB (X2) and total lipid concentration (X3). The optimization procedure generated the maximum overall desirability value. Central composite design succeeded in optimization of the formulation ingredients on the entrapment efficiency and in vitro release of Ketoprofen niosomes. Response surface methodology gave a mean to understand the effect of variables for the development of Ketoprofen niosomes. Finally the optimization process provides a formula having optimum level of factors as 0.66:1 from X1, 7.86 from X2, and 34.18 from X3. This optimized formula produces entrapment efficiency (Y1)equal to 42.22 % and release after 1 h (Y2), 6 h (Y3), and 12 h (Y4), 28.89 %, 71.64 % and 91.31 % respectively and these observed values of the optimized formula were close to the predicted values. Our study proved that experimental design methodology could efficiently be applied for characterization and optimization of formulation parameters affecting entrapment efficiency and drug release from ketoprofen niosomes.