Characterization of the Testicular Cell Types Present in the Rat by in Vivo 31P Magnetic Resonance Spectroscopy

Jeroen Van Der Grond, Ans M. M. Van Pelt, Cees J. A. Van Echteld, Grietje Dukstra, J. Anton Grootegoed, Dirk G. De Rooij, Willem P. Th. M. Mali
1991 Biology of Reproduction  
Testes of vitamin A-deficient Wistar rats before and after vitamin A replacement, of rats irradiated in utero, and of control rats were investigated by in vivo #{176}'P magnetic resonance (MR) spectroscopy. The testicular phosphomonoester/ATP (PM/ATP) ratio ranged from 0.79 ± 0.05 for testes that contained only interstitial tissue and Sertoli cells to 1.64 ± 0.04 for testes in which spermatocytes were the most advanced cell types present. When new generations of spermatids entered the
more » ... us epithelium, this ratio decreased. The testicular phosphodiester/ATP (PD/ATP) ratio amounted to 0.16 ± 0.06 for testes in which Sertoli cells, spermatogonla, or spermatocytes were the most advanced cell type present. When new generations of spermatids entered the seminiferous epithelium, the PD/ATP ratio rapidly increased and finally reached a value of 0.71 ± 0.06 for fully developed testes. Taken together, specific patterns of the PM/ATP ratio, the PD/ATP ratio, and pH were obtained that were correlated to the presence of spermatogonia, spermatocytes, round spermatids, and elongated spermatids or to the absence of spermatogenic cells, Hence, a good impression of the status of the seminiferous epithelium in the rat can be obtained by in vivo 'P MR spectroscopy.
doi:10.1095/biolreprod45.1.122 pmid:1652290 fatcat:eqsk6a5gbbeedop35bhxxximwq