Reactivity of Urinary Albumin (Microalbumin) Assays with Fragmented or Modified Albumin

D. Sviridov, S. K. Drake, G. L. Hortin
2007 Clinical Chemistry  
BACKGROUND: Controversy exists regarding occurrence and measurement of structural variants of albumin in urine. In this study, we examined cross-reactivity of in vitro modified albumins in assays for urine albumin (microalbumin). METHODS: We analyzed albumin modified by reagents, trypsin, or physical treatments or differing in primary sequence (animal albumins) with an immunoturbidimetric assay (Beckman LX20) using goat antiserum and a competitive immunoassay (Siemens Immulite) using a
more » ... l antibody. We assessed occurrence of albumin fragments in urine by use of Western blotting of 24 specimens.
doi:10.1373/clinchem.2007.092825 pmid:17981922 fatcat:ge4iepzg25grnggt7qun26oy3y