Bioinformatic tools for analysis of CLIP ribonucleoprotein data

Supriyo De, Myriam Gorospe
2016 Wiley Interdisciplinary Reviews - RNA  
Investigating the interactions of RNA-binding proteins (RBPs) with RNAs is a complex task for molecular and computational biologists. The molecular biology techniques and the computational approaches to understand RBP-RNA (or ribonucleoprotein, RNP) interactions have advanced considerably over the past few years and numerous and diverse software tools have been developed to analyze these data. Accordingly, laboratories interested in RNP biology face the challenge of choosing adequately among
more » ... available software tools those that best address the biological problem they are studying. Here, we focus on state-of-the-art molecular biology techniques that employ crosslinking and immunoprecipitation (CLIP) of an RBP to study and map RNP interactions. We review the different software tools and databases available to analyze the most widely used CLIP methods, HITS-CLIP, PAR-CLIP, and iCLIP.
doi:10.1002/wrna.1404 pmid:28008714 pmcid:PMC5509467 fatcat:mfgrjjvghjd6pjiryw2w5lomka