Dextran-Coated Charcoal Technique to Make the Hormone-Free Serum as a Diluent for Standard Curve of Radioimmunoassay

YUKIO OCHI, KATSUHIKO SHIOMI, TAKASHI HACHIYA, MANABU YOSHIMURA, TADAYOSHI MIYAZAKI
1973 Endocrinologia Japonica  
Synopsis Experiments were designed to make the hormone-free serum to serve for a standard curve of radioimmunoassay. The adsorption rate of hormone by dextran-coated charcoal (D.C.C.) was analysed using radioactive hormones such as 3, 5, 3' triiodothyronine(T3), thyroxine(T4), insulin, corticosterone, angiotensin I and II, ACTH, growth hormone(GH), TSH and HCG in pooled human serum. In general, the hormone of a small molecular weight was easily adsorbed into D.C.C., but the hormone of a large
more » ... lecular weight needed a large amount of D.C.C. to remove hormone. Using the solution of high concentrated D.C.C. (20% charcoal and 2% dextran), T3, insulin, corticosterone and angiotensin I and II were adsorbed by more than 80% into a small amount of D.C.C. solution (0.1ml), T4, GH and ACTH were adsorbed by more than 80% into a relatively large amount of D.C.C. solution (1ml), and TSH and HCG were removed by 75% into a greater amount of D.C.C. solution (2ml) under 1ml of pooled serum. In this treatment of D.C.C. solution, serum albumin was removed slightly into charcoal. The standard curve of radioimmunoassay for TSH and GH was figured using the panhypopituitary patient serum (PPS) or
doi:10.1507/endocrj1954.20.1 pmid:4353249 fatcat:rgpir4wxh5cfrlnxib4z7xhjue