N.gonorrhoeae strains which had newly-acquired TEM-type 8-lactamaseproducing ability were characterized for plasmid content and antimicrobial susceptibility. Enzyme production was found to be encoded by two species of nonconjugative plasmids of 3.2 and 4.4 Mdalton. Antibiotic susceptibility profiles of gonococcal strains harbouring the 3.2 Mdalton R-plasmid revealed the dissemination of a single clone. Permeability properties of strains harbouring the R-plasmid indicated that the plasmid did

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... contribute to intrinsic resistance in N.gonorrhoeae. The epidemiology, origin and transfer of these R-plasmids is discussed. High-level streptomycin resistance (MIC > 5,000 pg/ml) was chromosomalencoded and, using a poly(U)-direct ed cell-free protein synthesis system, EIP EDP-1] a) Energy requirements b)