Dye-coupled electrode system for the rapid determination of cell populations in polluted water

S Nishikawa, S Sakai, I Karube, T Matsunaga, S Suzuki
1982 Applied and Environmental Microbiology  
We determined cell populations in polluted waters by using a fuel cell-type electrode. The electrode was constructed from a platinum anode, a silver peroxide cathode, and a membrane filter for retaining microorganisms. The principle of cell number determination is based on sensing a redox dye reduced by the microorganisms with the electrode. Sample solutions containing microorganisms were membrane filtered, and the resulting filter containing microbial cells was attached to the surface of a
more » ... inum anode. The electrode was immersed in phosphate buffer solution (0.05 M, pH 7) containing a redox dye (2,4-dichlorophenolindophenol), and the current generated was measured. The response time of the electrode system was 10 to 20 min, and the current generated was proportional to cell populations above 104 cells/ml.
doi:10.1128/aem.43.4.814-818.1982 fatcat:tioioddwhbcvtc6dqeqaqkfexy